The attenuating effect of tolfenamic acid on nuclear factor kappa B gene expression in the seizures model of the male Wistar rats
کد: G-1041
نویسندگان: Kiyana Rahimi ℗, Mahyar Abdi, Narjes Askari, Mobina Salehi, Saba Fallahi, Atefeh Eshgevaryan, Saghi Hakimi Naeini, Abdolkarim Hosseini, Vahid Azizi ©
زمان بندی: زمان بندی نشده!
دانلود: دانلود پوستر
خلاصه مقاله:
background
Abnormal electrical activity in the brain leads to seizures, which manifest as unusual behaviors. One of the primary causes of seizures is neuroinflammation, largely driven by the activation of a protein called nuclear factor kappa B (NF-κB). This research examines how tolfenamic acid (TA) affects seizures in Wistar rats when the seizures are triggered by pentylenetetrazol (PTZ).
Methods
The rats were subjected to PTZ-induced seizures at dose of 60 mg/kg and were randomly divided into four groups, each consisting of 5 rats (weighing 200-250 g). The groups included: a healthy control, an untreated PTZ control group (which received saline 30 minutes before PTZ), and two treatment groups that received TA at doses of 10 and 50 mg/kg. Brain cell samples were collected to evaluate the effect of TA on the expression of NF-κB gene using Real-time PCR.
Findings
The results showed that The PTZ group, which did not receive TA, displayed a pronounced inflammatory response, characterized by significantly augmented NF-κB expression compared to the control group (P0.01). Conversely, animals administered TA exhibited a marked attenuation of NF-κB expression relative to the PTZ group (P0.05).
Conclusion
These findings suggest that TA could be considered a potential therapeutic option for controlling inflammation-related seizures through attenuation of the NF-κB gene.
Key words
Tolfenamic Acid, NF-κB,
